Coadministration of Alpha Tocopherol & Selenium; A Possible Intervention to Attenuate Amikacin Induced Nephrotoxicity in Rabbits

Abstract

The study was designed to evaluate the nephroprotective effect of alpha tocopherol and selenium (TS) in amikacin (A) induced nephrotoxity in rabbits. 24 male rabbits were randomly divided into four groups; 6 animals in each group. Group A received amikacin sulphate 150mg/ kg b.w. Group ATS was given amikacin sulphate 150 mg/kg body weight co-administered with alpha tocopherol 1 g/kg dissolved in olive oil and selenium 2mg/kg as sodium salinities where as Group TS was put on alpha tocopherol 1g/kg b.w and selenium as sodium salenite 2mg/kg b.w alone, while control group being on simple isotonic saline. All the medications continued for 20 days intramuscularly in two divided doses. Twenty four hours urine and blood samples were collected at 0 (before therapy), 11th and 21st days of study period. Blood samples were analyzed for BUN, creatinine, glucose & creatinine clearance where as urine samples were used for estimation of pH, sugar & protein. The nephrotoxic results revealed that amikacin enhanced the levels of BUN and creatinine, while reducing the creatinine clearance along with significant glucosuria and proteinuria. But when alpha tocopherol & selenium were coadministered with amikacin, the results showed a change in biochemical markers towards normal. It is concluded that co administration of alpha tocopherol and selenium alleviated amikacin induced nepherotoxity due to their antioxidant potentials.